Download A Manual of Chemical & Biological Methods for Seawater by Timothy Richard Parsons PDF
By Timothy Richard Parsons
An advent to the quantitative research of seawater, describing intimately organic and chemical ideas, that are thought of to be among these in most cases utilized by organic oceanographers. The guide offers whole directions for the addition of reagents and calculation of effects with regard fabric for every process in order that the unique texts may be consulted if valuable. ordinarily, the innovations require no less than past expert education and strategies wanting very pricey apparatus were refrained from
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Additional info for A Manual of Chemical & Biological Methods for Seawater Analysis
Notes (a) A method for determining very low levels of phosphate, particularly such as are encountered in some tropical waters, is given by Stephens (Limnol. , 8: 361, 1963). The method is the same as that described here except that the blue color produced is concentrated by extraction with iso-butanol and measured at 690 nm. The method results in an approximate 5-fold increase in sensitivity. (b) If high blank values are experienced, the a m m o n i u m molybdate should be made up again and tested.
25 g of analytical grade sodium arsenite in 100 ml of redistilled water. The solution is stable indefinitely. 4. Ferric chloride solution Dissolve 5 g of analytical grade ferric chloride in 100 ml of redistilled water, filter through a W h a t m a n G F C filter and store at 5°C. 5. 1 N HC1 with warming; filter through a glass fiber filter if precipitates remain. Store in a clean amber bottle at room temperature. This solution should be prepared fresh every day. 6. Acetone Use analytical grade acetone.
To a 100 ml sample, add 10 ml of mixed reagent using a syringe-type pipette and mix at once. 3. After 5 min, and preferably within the first 2-3 h (Note d), measure the extinction in a 10-cm cell against distilled water at 885 nm. 24 Nutrients 4. Correct the extinction with the reagent blank (and turbidity blank if necessary) and calculate the phosphate concentration as: /ig-at P/l = corrected extinction x F where F is the factor as described below. G. Determination of blank Use distilled water in place of a sample and carry out steps 1-4 above to obtain the extinction of the reagent blank.