Download Algal Culturing Techniques by Robert A. Andersen PDF
By Robert A. Andersen
Algal Culturing Techniques is a entire reference on all elements of the isolation and cultivation of marine and freshwater algae, together with seaweeds. it really is divided into seven components that disguise heritage, media guidance, isolation and purification thoughts, mass culturing strategies, phone counting and development size innovations, and experiences on themes and functions of algal tradition recommendations for environmental investigations.
Algal Culturing Techniques used to be built to function either a brand new textbook and key reference for phycologists and others learning aquatic structures, aquaculture and environmental sciences. scholars of algal ecology, marine botany, marine phycology, and microbial ecology will benefit from the hands-on method for culturing numerous algae from clean and marine waters. Researchers in undefined, akin to aquaculture, pharmaceutical, foodstuffs, and biotechnology businesses will locate an authoritative and complete reference.
- Sponsored through the Phycological Society of America
- Features colour images and illustrations throughout
- Describes culturing tools starting from the try out tube to outdoors ponds and coastal seaweed farms
- Details isolation strategies starting from conventional micropipette to automatic move cytometeric methods
- Includes purification, progress, upkeep, and cryopreservation techniques
- Highlights tools for estimating algal populations, development premiums, keeping apart and measuring algal pigments, and detecting and culturing algal viruses
- Features a finished appendix of approximately 50 algal tradition medium recipes
- Includes a word list of phycological terms
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Extra info for Algal Culturing Techniques
R. 1957. The development of artiﬁcial media for marine algae. Arch. Mikrobiol. 25:392–428. , and Hepperle, D. 2000. Variability of calciﬁcation of Phacotus lenticularis (Chlorophyta, Chlamydomonadales) in nature and culture. Phycologia 39:318–22. Schlösser, U. G. 1994. SAG—Sammlung von Algenkulturen at the University of Göttingen catalogue of strains 1994. Bot. Acta 107:111–86. , and Nakano, M. 1989. Development of a solid medium for growth and isolation of axenic Microcystis strains (cyanobacteria).
The reader is cautioned that the Web site recipes are subject to change; indeed, Berges et al. (2001) recommended that Web-based recipes may have real advantages for phycological research. In both AW and NW recipes, there is some variation in salinity. McLachlan (1973) considered that salinity was not an “inherent feature” of media recipes. Although salinities of 35 are often considered normal, most artiﬁcial media tend to produce somewhat lower salinities (27 to 30). Many coastal phytoplankton species can be cultured at a much lower salinity (cf.
7. Sterilizing smaller volumes of seawater with a microwave (Keller et al. 1988). 8. Lowering the concentration of iron. 0. 1. Macronutrients (Nitrogen, Phosphorus, and Silicon) Macronutrients are generally considered to be nitrogen, phosphorus, and silicon. However, silicon is required only for diatoms, silicoﬂagellates, and some chrysophytes. These macronutrients are generally required in 26 Marine Culture Media a ratio of 16N:16Si:1P (Parsons et al. 1984, Brzezinski 1985), and the ambient ratio in NW is often similar to the ratio required by the algae, except in some estuaries where there are large inputs of nitrogen and phosphorus.