Download BSL3 and BSL4 Agents: Epidemiology, Microbiology, and by Mandy Elschner, Sally Cutler, Manfred Weidmann, Patrick PDF
By Mandy Elschner, Sally Cutler, Manfred Weidmann, Patrick Butaye
In a single convenient booklet, this reference gathers the entire worthy details on 14 of the main familiar risky teams of pathogens in biosafety point three and four laboratories. the entire chapters are uniformly based, with a quick review of the microbiology, pathology, epidemiology and detection equipment for every crew. additionally, a complete bankruptcy is dedicated to the precise biosafety specifications, disinfection, decontamination protocols, twist of fate literature and twist of fate systems, in addition to cures for all of the organisms. This bankruptcy is obviously marked and simple to discover whilst starting the book.
crucial literature for the expanding variety of BSL3 or BSL4 labs around the globe and for clinical amenities intervening in relation to an incident.Content:
Chapter 1 Bacillus anthracis: Anthrax (pages 1–18): Markus Antwerpen, Paola Pilo, Pierre Wattiau, Patrick Butaye, Joachim Frey and Dimitrios Frangoulidis
Chapter 2 Brucella Species: Brucellosis (pages 19–35): Sally J. Cutler, Michel S. Zygmunt and Bruno Garin?Bastuji
Chapter three Burkholderia mallei: Glanders (pages 37–46): Lisa D. Sprague and Dr. Mandy C. Elschner
Chapter four Burkholderia pseudomallei: Melioidosis (pages 47–56): Lisa D. Sprague and Dr. Mandy C. Elschner
Chapter five Coxiella burnetii: Q Fever (pages 57–69): Matthias Hanczaruk, Dr. Sally J. Cutler, Rudolf Toman and Dimitrios Frangoulidis
Chapter 6 Francisella Tularensis: Tularemia (pages 71–84): Anders Johansson, Herbert Tomaso, Plamen Padeshki, Anders Sjostedt, Nigel Silman and Paola Pilo
Chapter 7 Yersinia Pestis: Plague (pages 85–121): Anne Laudisoit, Werner Ruppitsch, Anna Stoeger and Ariane Pietzka
Chapter eight Rickettsia Species: Rickettsioses (pages 123–148): Alice N. Maina, Stephanie Speck, Eva Spitalska, Rudolf Toman, Gerhard Dobler and Dr. Sally J. Cutler
Chapter nine Mycobacterium Tuberculosis: Tuberculosis (pages 149–171): Stefan Panaiotov, Massimo Amicosante, Marc Govaerts, Prof. Patrick Butaye, Elizabeta Bachiyska, Nadia Brankova and Victoria Levterova
Chapter 10 Influenza Virus: hugely Pathogenic Avian Influenza (pages 173–200): Chantal J. Snoeck, Nancy A. Gerloff, Radu I. Tanasa, F. Xavier Abad and Claude P. Muller
Chapter eleven Variola: Smallpox (pages 201–210): Andreas Nitsche and Hermann Meyer
Chapter 12 Arenaviruses: Hemorrhagic Fevers (pages 211–235): Amy C. Shurtleff, Steven B. Bradfute, Sheli R. Radoshitzky, Peter B. Jahrling, Jens H. Kuhn and Sina Bavari
Chapter thirteen Filoviruses: Hemorrhagic Fevers (pages 237–252): Victoria Wahl?Jensen, Sheli R. Radoshitzky, Sina Bavari, Peter B. Jahrling and Jens H. Kuhn
Chapter 14 Bunyavirus: Hemorrhagic Fevers (pages 253–289): Ali Mirazimi, Dr. Manfred Weidmann, F. Xavier Abad, Janusz T. Paweska and Jonas Klingstrom
Chapter 15 Bacillus anthracis (pages 291–297): Markus Antwerpen, Paola Pilo, Pierre Wattiau, Prof. Patrick Butaye, Joachim Frey and Dimitrios Frangoulidis
Chapter sixteen Brucella Species (pages 298–300): Dr. Sally J. Cutler, Michel S. Zygmunt and Bruno Garin?Bastuji
Chapter 17 Burkholderia mallei: Glanders (pages 301–302): Lisa D. Sprague and Dr. Mandy C. Elschner
Chapter 18 Burkholderia pseudomallei: Melioidosis (pages 303–305): Lisa D. Sprague and Dr. Mandy C. Elschner
Chapter 19 Coxiella burnetii: Q Fever (pages 306–308): Matthias Hanczaruk, Dr. Sally J. Cutler, Rudolf Toman and Dimitrios Frangoulidis
Chapter 20 Francisella tularensis: Tularemia (pages 309–311): Anders Johansson, Herbert Tomaso, Plamen Padeshki, Anders Sjostedt, Nigel Silman and Paola Pilo
Chapter 21 Yersinia pestis: Plague (pages 312–317): Anne Laudisoit, Werner Ruppitsch, Anna Stoeger and Ariane Pietzka
Chapter 22 Rickettsia Species: Rickettsioses (pages 318–321): Alice N. Maina, Stephanie Speck, Eva Spitalska, Rudolf Toman, Gerhard Dobler and Sally J. Cutler
Chapter 23 Mycobacterium tuberculosis: Tuberculosis (pages 322–325): Stefan Panaiotov, Massimo Amicosante, Marc Govaerts, Prof. Patrick Butaye, Elizabeta Bachiyska, Nadia Brankova and Victoria Levterova
Chapter 24 Influenza Virus: hugely Pathogenic Avian Influenza (pages 326–333): Chantal J. Snoeck and Claude P. Muller
Chapter 25 Variola: Smallpox (pages 334–337): Andreas Nitsche and Hermann Meyer
Chapter 26 Arenaviruses: Hemorrhagic Fevers (pages 338–343): Amy C. Shurtleff, Steven B. Bradfute, Sheli R. Radoshitzky, Peter B. Jahrling, Jens H. Kuhn and Sina Bavari
Chapter 27 Filoviruses: Hemorrhagic Fevers (pages 344–349): Victoria Wahl?Jensen, Sheli R. Radoshitzky, Sina Bavari, Peter B. Jahrling and Jens H. Kuhn
Chapter 28 Bunyavirus (pages 350–360): Ali Mirazimi, Dr. Manfred Weidmann, F. Xavier Abad, Janusz T. Paweska and Jonas Klingstrom
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Additional resources for BSL3 and BSL4 Agents: Epidemiology, Microbiology, and Practical Guidelines
The results of such tests can be used to trace back the origin of the investigated isolates. In variable number tandem repeats (VNTRs), multi-locus variable analysis (MLVA), or single nucleotide repeat (SNR) analysis, the gene loci comprising repeated motifs bordered by conserved DNA regions are investigated. The combined lengths of the examined loci are likely to differ from strain to strain. The length of the repeated units varies from one base in the case of SNR to several dozens (macro-satellites) in B.
Anthracis from B. cereus, while phage testing with bacteriophage gamma is more speciﬁc. The feature of sensitivity of B. anthracis to gamma phage is still used for identiﬁcation although exceptions were described in this case, too. In former times veterinary laboratories used a mouse inoculation test for conﬁrmation. When all phenotypic characteristics were present and the phage test was positive, and ﬁnally the mouse died within one day after intraperitonal inoculation, and bacillus-like bacteria were seen on colored blood samples, the isolated bacterium was seen as a virulent strain of B.
As an alternative, trimethoprim-sulfamethoxazole-polymyxin-blood agar (TSPB agar) can be used, containing several antibiotics and resulting in a high selectivity against Gram-negative bacteria. 1 Spike-like projections at the colony edge from Bacillus anthracis (in addition no hemolysis on Columbia agar; Bundeswehr Institute of Microbiology, Munich, Germany). 2 ‘‘Sticky colony’’ phenomenon of B. anthracis after manipulation with a loop (J. Frey, Bern, Switzerland). Even if only some of these features are observed, the results should be considered as suspicious for B.